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CABRI:ICLC |
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Accession_number |
ICLC HTL08002
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Cell_line_name |
A2058
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Brief_description |
Species: human, Caucasian; Tissue: skin; Tumor: melanoma, metastatic; Derived from: lymph node metastasis
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Description |
Species: human, Caucasian male 43 years old; Tissue: skin; Tumor: melanoma, metastatic; Derived from: lymph node metastasis
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Depositor |
Dr F. Tosetti, Cellular Biology Dept, IST at ABC, Genoa, Italy
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Bibliographic_reference |
-
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Morphology |
epithelial, grown as monolayer
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Culture_conditions |
continuous culture; RPMI 1640 + 10% FBS + 2mM-Glutamine + 4,5 g/L glucose; split confluent cultures1:3-1:6; 37C, 5% CO2
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Viruses |
Search for viruses was not performed
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Release_conditions |
Cell line available for distribution. For non-commercial investigative use only
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Hazard |
-
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Freezing_medium |
Culture medium 45% + 50% FCS + 5% DMSO
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Sterility |
mycoplasma negative, HOECHST and PCR
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Validation_assays |
Multiplex PCR: tested against human, rat, mouse primers. Confirmed as human with cytochrome c oxidase subunit I (cox I) primers
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Further_bibliography |
Proc Natl Acad Sci U S A. 1977;74(2):565-9 [PMID: 265522
]
Proc Natl Acad Sci U S A. 1979; 76(3):1288-92 [PMID: 375235
]
Proc Natl Acad Sci U S A. 1980;77(9):5258-62 [PMID: 6254071
]
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